Association of microRNAs genes polymorphisms with rheumatoid arthritis in Egyptian female patients

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• 作者：Amal S. El-Shal ; Nader M. Aly ; Sahar M. Abdel Galil ; Mohamed A. Moustafa ; Wael A. K ; el
• 关键词：Rheumatoid arthritis ; MicroRNA ; Polymorphism ; Egyptian population
• 刊名：Joint Bone Spine
• 出版年：December, 2013
• 年：2013
• 卷：80
• 期：6
• 页码：626-631
• 全文大小：548 K

文摘

Objective

To investigate whether miRNA-499 (rs3746444) and miRNA-146a (rs2910164) genes polymorphisms are independent factors for rheumatoid arthritis (RA) in Egyptians, and whether they influence disease severity and activity.

Methods

Two hundred and seventeen RA patients and 245聽healthy controls were enrolled in this study. Polymorphisms of miRNA-146a and miRNA-499 genes were detected using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).

Results

The miRNA-499 CT genotype was an independent factor of RA. The miRNA-499 CT, CC genotypes and C allele frequencies were significantly increased in erosive RA group. Moreover, the heterozygote CT had more severe and more active form of the disease compared with homozygote CC or TT. However, we did not find any significant association of miRNA-146a polymorphism with RA risk, severity, and activity.

Conclusion

The miRNA-499 polymorphism is an independent factor of RA, and influences disease severity and activity.