Investigation of gold nanoparticles uptake and their tissue level distribution in rice plants by laser ablation-inductively coupled-mass spectrometry

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• 作者：Jeremy Koelmel^a ; Thomas Leland^a ; Huanhua Wang^b ; Dulasiri Amarasiriwardena^a ; ^{dans@hampshire.edu} ; Baoshan Xing^b
• 关键词：Gold nanoparticle ; Uptake ; LA-ICP-MS ; Rice (Oryza sativa L.) ; Bioimaging
• 刊名：Environmental Pollution
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：174
• 期：Complete
• 页码：222-228
• 全文大小：807 K

文摘

The tissue level uptake and spatial distribution of gold nanoparticles (AuNPs) in rice (Oryza sativa L.) roots and shoots under hydroponic conditions was investigated using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Rice plants were hydroponically exposed to positively, neutrally, and negatively charged AuNPs [AuNP1(+), AuNP2(0), AuNP3(?)] with a core diameter of 2?nm. Plants were exposed to AuNPs having 1.6?mg?Au/L for 5 days or 0.14?mg?Au/L for 3 months to elucidate how the surface charges of the nanoparticles affects their uptake into living plant tissues. The results demonstrate that terminal functional groups greatly affected the AuNP uptake into plant tissues. Au concentration determined by LA-ICP-MS in 5 day treated rice roots followed this order: AuNP1(+)?>?AuNP2(0)?>?AuNP3(?) but this order was reversed for rice shoots, indicating preferential translocation of AuNP3(?). Bioimages revealed distributions of mesophyll and vascular AuNP dependent on organ or AuNP concentration.