Micro-scale flow system for on-line multielement preconcentration from saliva digests and determination by inductively coupled plasma optical emission spectrometry

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• 作者：Meneg&aacute ; rio ; Amauri A. ; Fernanda Giné ; Maria
• 关键词：Saliva analysis ; Inductively coupled plasma optical emission spectrometry ; Micro-concentric nebulizer ; Miniaturized flow system
• 刊名：Spectrochimica Acta Part B: Atomic Spectroscopy
• 出版年：2001
• 出版时间：October 31, 2001
• 年：2001
• 卷：56
• 期：10
• 页码：1917-1925
• 全文大小：178 K

文摘

A micro-scale flow system is proposed for on-line preconcentration of Cd, Cu, Mn, Ni and Pb in saliva samples and their determination by inductively coupled plasma optical emission spectrometry (ICP-OES). A small column containing 8 μl of AG50W-X8 resin was inserted into the flow system, assembled with capillary tubes and connected to a micro-concentric nebulizer. The elution of the analytes was performed with 3 mol l⁻¹ HCl at a flow rate of 82 μl min⁻¹. The ICP-OES signal acquisition program permits measurements for 5 s in the concentrated portion of the transient elution peaks. A sample volume of 1 ml was required to obtain enrichment factors of 46, 23, 17, 18 and 44 for Cd, Cu, Mn, Ni and Pb, respectively. The relative standard deviations for a 50-μg l⁻¹ multi-analyte solution were ≤6.5 % . The recoveries for Cd, Cu, Mn, Ni and Pb in digested human saliva samples were between 86 and 111 % . The sample throughput was 24 h⁻¹.