Simple multiresidue extraction method for the determination of fungicides and plant growth regulator in bean sprouts using low temperature partitioning and tandem mass spectrometry

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• 作者：Soon-Kil Cho^a ; A.M. Abd El-Aty^b ; ^{abdelaty44@hotmail.com} ; Ki Hun Park^c ; Jong-Hyouk Park^c ; M.E. Assayed^d ; Yang-Mo Jeong^a ; Young-Seok Park^a ; Jae-Han Shim^c ; ^{jhshim@chonnam.ac.kr}
• 关键词：Bean sprouts ; Plant growth regulator ; Fungicides ; Multiresidue analysis ; Tandem mass spectrometry
• 刊名：Food Chemistry
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：136
• 期：3-4
• 页码：1414-1420
• 全文大小：812 K

文摘

A simple multiresidue analytical method is developed for the simultaneous determination of carbendazim (CB), thiabendazole (TB), and 6-benzyl aminopurine (6-BA) in bean sprouts. The samples were extracted with acetonitrile followed by partitioning at ?80 ¡ãC for 5-10 min. A YMC C₈ column was used to separate the analytes before being qualitatively and quantitatively determined by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in positive ion mode using multiple reaction monitoring (MRM). The matrix-matched calibration curves showed good linearity in the range 0.01-1.0 mg/kg with correlation coefficients in excess of 0.998. The mean recoveries were in the range of 80.4-96.3 % at 0.1 and 0.5 spiked levels, and the relative standard deviations (RSDs) were in the range of 0.5-7.6 % . The limits of quantifications (LOQ) were in the range of 0.005-0.01 mg/kg. The method was successfully applied to 90 samples (among which 45 were organic) collected from a commercial bean sprout production house throughout the city. Except for 6-BA, the rest of the analytes had values lower than their LOQs. In sum, carbendazim, thiabendazole, and 6-BA were extracted in a single step, and no steps for clean-up or concentration of the extracts were needed. The current method can be used for sensitive and accurate determination and confirmation of residues in bean sprout samples.