Taxol and 10-deacetylbaccatinIII induce distinct changes in the dynamics of caveolae

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• 作者：Neesar Ahmed ; Sreekanth Dasari ; Saumya S. Srivastava ; Amita Sneh ; Absar Ahmad ; M. Islam Khan ; M.V. Krishnasastry
• 关键词：Caveolae ; Kiss and run dynamic ; TIRFM ; Taxol ; 10-DeacetylbaccatinIII ; BaccatinIII ; Cytotoxicity
• 刊名：FEBS Letters
• 出版年：2008
• 出版时间：29 October 2008
• 年：2008
• 卷：582
• 期：25-26
• 页码：3595-3600
• 全文大小：2199 K

文摘

Taxol treatment of HeLa cells resulted in a transient recruitment of Caveolin-1 to the cell surface followed by internalization. Interestingly, 20 min after 10-deacetylbaccatinIII (10-DAB) treatment, the caveolae displayed faster ‘kiss and run’ dynamics while BaccatinIII (BacIII) did not induce any change. Sustained phosphorylation of Caveolin-1 is observed upon treatment and between Taxol and 10-DAB, the former shows phosphorylated Raf-1, ERK1/2 and hyperphosphorylated Bcl-2 while the later showed much less magnitude of the same. BacIII treatment did not induce phosphorylation of Raf-1 or Bcl-2. It is possible that Taxol might act on multiple targets and the side chain may be crucial.