Determination of four sulfated vitamin D compounds in human biological fluids by liquid chromatography-tandem mass spectrometry
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- 作者：Fabio P. Gomes ; P. Nicholas Shaw ; Amitha K. Hewavitharana ; a.hewavitharana@pharmacy.uq.edu.au" class="auth_mail" title="E-mail the corresponding author
- 关键词：Vitamin D sulfate ; Water-soluble vitamin D ; Breastmilk ; Human serum ; Electrospray ionisation
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：1009-1010
- 期：Complete
- 页码：80-86
- 全文大小：1103 K
文摘
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Concurrent quantification of four water-soluble (sulfated) vitamin D forms.
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Evaluation of ESI positive and negative modes to obtain femtomole sensitivity.
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Correction for matrix effects in MS detector through internal standardization.
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Validation of the method prior to analysis of human serum and breastmilk.