Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry to differentiate between Candida albicans and Candida dubliniensis
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- 作者：Amity L. Robertsa ; amity.roberts@jefferson.edu" class="auth_mail" title="E-mail the corresponding author ; Aqilah Alelewd ; aqela4@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Peter C. Iwenb ; c ; piwen@unmc.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：MALDI-TOF-MS ; Candida dubliniensis ; ITS1 ; ITS4
- 刊名：Diagnostic Microbiology & Infectious Disease
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：85
- 期：1
- 页码：73-76
- 全文大小：260 K
文摘
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Evaluation of a well-characterized strain set of Candida albicans and Candida dubliniensis showed that matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF-MS), when using the direct formic acid (FA) extraction method, was able to accurately discriminate between C. albicans and C. dubliniensis.
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Utilization of this technology will help to define the true epidemiological prevalence of C. dubliniensis and expand knowledge of what role this species contributes to human infection.
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Direct FA method for processing Candida species for MALDI-TOF-MS analysis is as effective as a traditional ethanol extraction process.