Intraspecies variability in Vipera ammodytes ammodytes venom related to its toxicity and immunogenic potential

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• 作者：Beata Halassy^a ; ^{bhalassy@imz.hr} ; Marija Brgles^a ; Lidija Habjanec^a ; Maja Lang Balija^a ; Tihana Kurtović ; ^a ; Martina Marchetti-Deschmann^b ; Igor Križ ; aj^c ; Gü ; nter Allmaier^b
• 关键词：Vipera ammodytes ammodytes venom ; Ammodytoxin ; Monolithic columns ; Capillary electrophoresis-on-a-chip ; ELISA ; Lethal toxicity
• 刊名：Comparative Biochemistry and Physiology, Part C
• 出版年：2011
• 出版时间：March 2011
• 年：2011
• 卷：153
• 期：2
• 页码：223-230
• 全文大小：1183 K

文摘

Vipera ammodytes is the most venomous European snake, whose venom has been used as antigen for immunization of antivenom-producing animals. Same as venom of any other snake, it is a complex mixture of proteins, peptides and other compounds which biochemical and pharmacological variability has been demonstrated at interspecies and intraspecies level. In this work we demonstrated intraspecific variability between 8 venom production batches using both the conventional and the new methodology. Moreover, in contrast to the literature on different venoms' variability, for the first time we were able to select those biochemical differences that are related to and give information on the venom's toxicity and immunogenicity. We have shown that methods quantifying ammodytoxin (the most toxic compound identified so far in the Vipera ammodytes ammodytes venom) content of the venom clearly distinguish between high and low immunogenic venoms.