刊名:Biochimica et Biophysica Acta (BBA) - General Subjects
出版年:2017
出版时间:February 2017
年:2017
卷:1861
期:2
页码:450-456
全文大小:703 K
卷排序:1861
文摘
A set of engineered archael ferritin mutants have been obtained. The mutants bear cysteine thiols in selected topological positions. Thiol reactivity has been probed using the bulky thiol reactive DTNB. Archaeal ferritins can bind DTNB outside but also inside the cavity. Protein matrix is not a significant barrier against bulky, negatively charged ligands.