Isothermal amplification detection of nucleic acids by a double-nicked beacon

详细信息    查看全文

• 作者：Chao Shi^a ; Meiling Zhou^a ; Mei Pan^b ; Guilin Zhong^b ; Cuiping Ma^b ; ^{mcp169@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ssDNA detection ; Isothermal amplification ; Double-nicked beacon ; Cascade amplification ; Real-time fluorescence
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：1 March 2016
• 年：2016
• 卷：496
• 期：Complete
• 页码：9-13
• 全文大小：1160 K

文摘

Isothermal and rapid amplification detection of nucleic acids is an important technology in environmental monitoring, foodborne pathogen detection, and point-of-care clinical diagnostics. Here we have developed a novel method of isothermal signal amplification for single-stranded DNA (ssDNA) detection. The ssDNA target could be used as an initiator, coupled with a double-nicked molecular beacon, to originate amplification cycles, achieving cascade signal amplification. In addition, the method showed good specificity and strong anti-jamming capability. Overall, it is a one-pot and isothermal strand displacement amplification method without the requirement of a stepwise procedure, which greatly simplifies the experimental procedure and decreases the probability of contamination of samples. With its advantages, the method would be very useful to detect nucleic acids in point-of-care or field use.