Target-triggered triple isothermal cascade amplification strategy for ultrasensitive microRNA-21 detection at sub-attomole level
详细信息 查看全文
- 作者：Fang-Fang Chengb ; c ; 1 ; Nan Jianga ; b ; 1 ; Xiaoyan Lia ; Li Zhangc ; Lihui Hub ; Xiaojun Chena ; chenxj_njut@126.com" class="auth_mail" title="E-mail the corresponding author ; Li-Ping Jiangb ; Jianglp@nju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; E.S. Abdel-Halimd ; Jun-Jie Zhub
- 关键词：Target-triggered triple amplification ; Isothermal cascade reaction ; Attomolar sensitivity ; microRNA-21 ; Electrochemical detection
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：85
- 期：Complete
- 页码：891-896
- 全文大小：1195 K
文摘
- •
Sensitive and selective detection of miR-21 is vital to the clinical diagnosis for breast cancer.
- •
A target-triggered triple isothermal cascade amplification strategy has been newly developed.
- •
A tri-functional DNA template contains target recognition, analogue amplification and DNAzyme production parts.
- •
Polymerase-nicking enzyme, 8-17 DNAzyme and HRP-mimicking DNAzyme were employed in the cascade reaction.
- •
Attomolar sensitivity with excellent selectivity for the quantification of miR-21 is realized.