An immunoassay-based reverse-transcription loop-mediated isothermal amplification assay for the rapid detection of avian influenza H5N1 virus viremia
详细信息 查看全文
- 作者：Yi Tanga ; Xu Yub ; Hao Chena ; Youxiang Diaoa ; yxdiao@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Avian influenza virus ; RT-LAMP ; H5N1 ; Viremia ; Immunoassay
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：255-261
- 全文大小：1888 K
文摘
- •
H5 monoclonal antibody-based virus capture and N1 gene based RT-LAMP for one-pot detection of H5N1.
- •
In-tube thermal viral RNA releasing is comparable to conventional RNA extraction assay.
- •
Integrated EvaGreen dye make result more easily to be observed by biosensors or naked eye.
- •
The developed Immuno-RT-LAMP strategy is capable to process the blood sample and detect the H5N1 viremia.