STR fragments obtained with the NGM SElect™ Kit and SNPs using a prototype version of the Ion AmpliSeq™ Identity panel (v2.3) with massive parallel sequencing was evaluated on LtDNA samples.
Sensitivity study was performed down to 5 pg with control-DNA and degradation studies were conducted with control DNA and blood reference samples. A total of 1900 independent genotypes were analyzed. Allele balance and consistency in the obtained genotypes were evaluated.
Casework samples were processed with Ion AmpliSeq™ Identity panel and Random Match Probability (RMP) was calculated in order to compare the Power of Identity (PI) for both the SNP and STR profiles.
On the basis of our evaluation, the prototype Ion AmpliSeq™ Identity panel v2.3 demonstrated to be a fast, robust and valuable tool for gathering identity information in challenging situations such as LtDNA.