- 作者：Salata Elenaa ; 1 ; elena.salata@carabinieri.it" class="auth_mail" title="E-mail the corresponding author ; Agostino Alessandrob ; 1 ; slessandro.agostino@thermofisher.com" class="auth_mail" title="E-mail the corresponding author ; Ciuna Ignazioa ; Wootton Sharonc ; sharon.wootton@thermofisher.com" class="auth_mail" title="E-mail the corresponding author ; Ripani Luigia ; Berti Andreaa
- 关键词：Ion AmpliSeq&trade ; Identity panel ; PGM&trade ; Sequencer ; Massive parallel sequencing ; STR analysis ; Quantifiler® ; LtDNA
- 刊名：Forensic Science International: Genetics
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：22
- 期：Complete
- 页码：25-36
- 全文大小：1458 K
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STR fragments obtained with the NGM SElect™ Kit and SNPs using a prototype version of the Ion AmpliSeq™ Identity panel (v2.3) with massive parallel sequencing was evaluated on LtDNA samples.
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Sensitivity study was performed down to 5 pg with control-DNA and degradation studies were conducted with control DNA and blood reference samples. A total of 1900 independent genotypes were analyzed. Allele balance and consistency in the obtained genotypes were evaluated.
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Casework samples were processed with Ion AmpliSeq™ Identity panel and Random Match Probability (RMP) was calculated in order to compare the Power of Identity (PI) for both the SNP and STR profiles.
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On the basis of our evaluation, the prototype Ion AmpliSeq™ Identity panel v2.3 demonstrated to be a fast, robust and valuable tool for gathering identity information in challenging situations such as LtDNA.