Physico-chemical characteristics and primary structure of an affinity-purified α-D-galactose-specific, jacalin-related lectin from the latex of mulberry (Morus indica)
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- 作者：Debparna Dattaa ; Gottfried Pohlentzb ; Mona Schulteb ; Mathias Kaiserc ; Francisco M. Goycooleac ; Johannes Mü ; thingb ; Michael Mormannb ; Musti J. Swamya ; mjswamy@uohyd.ac.in" class="auth_mail" title="E-mail the corresponding author ; mjswamy1@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Agglutinin ; gJRL ; Pauci-mannose-type glycan ; Mass spectrometry ; Cytotoxicity
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：1 November 2016
- 年：2016
- 卷：609
- 期：Complete
- 页码：59-68
- 全文大小：2007 K
文摘
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MLGL, a gJRL was purified from the latex of mulberry by affinity chromatography.
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MLGL is a homotetrameric glycoprotein and each subunit has two polypeptide chains.
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The heavier and lighter chains have 133 and 21 (or 24) amino acids, respectively.
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A pauci-mannose (or complex type) N-glycan is attached to Asn47 in the heavier chain.
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MLGL is cytotoxic towards MCF-7 and MDCK cells even at micromolar concentrations.