Expression of P2X₅ receptors in the mouse CNS

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• 作者：W. Guo ; X. Xu ; X. Gao ; G. Burnstock ; C. He ; Z. Xiang
• 关键词：P2X₅ receptor ; immunohistochemistry ; in situ hybridization ; CNS
• 刊名：Neuroscience
• 出版年：2008
• 出版时间：15 October 2008
• 年：2008
• 卷：156
• 期：3
• 页码：673-692
• 全文大小：7388 K

文摘

P2X receptors are ATP-gated cationic channels composed of seven known subunits (P2X_1-7) which are involved in different functions in neural tissue. The present study investigates the P2X₅ receptor expression pattern in the mouse CNS using immunohistochemistry and in situ hybridization histochemistry. The specificity of the immunostaining has been verified by pre-absorption, Western blot and in situ hybridization methods. Heavy P2X₅ receptor immunostaining was observed in the mitral cells of the olfactory bulb; cerebral cortex; globus pallidum, anterior cortical amygdaloid nucleus, amygdalohippocampal area of subcortical telencephalon; anterior nuclei, anteroventral nucleus, ventrolateral nucleus of thalamus; supraoptic nucleus, ventromedial nucleus, arcuate nucleus of hypothalamus; substantia nigra of midbrain; pontine nuclei, mesencephalic trigeminal nucleus, motor trigeminal nucleus, ambiguous nucleus, inferior olive, hypoglossal nucleus, dorsal motor vagus nucleus, area postrema of hindbrain; Purkinje cells of cerebellum; and spinal cord. The identification of extensive P2X₅ receptor immunoreactivity and mRNA distribution within the CNS of the mouse demonstrated here is consistent with a role for extracellular ATP acting as a fast neurotransmitter.