Radial derivatives of the mouse ventral pallium traced with Dbx1-LacZ reporters

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• 作者：Luis Puelles^a ; ¹ ; ^{puelles@um.es" class="auth_mail" title="E-mail the corresponding author} ; Loreta Medina^a ; ¹ ; ² ; Ugo Borello^b ; ³ ; Isabel Legaz^a ; ⁴ ; Anne Teissier^b ; ⁵ ; ^{anne.teissier@inserm.fr" class="auth_mail" title="E-mail the corresponding author} ; Alessandra Pierani^b ; John L.R. Rubenstein^c
• 关键词：Ventral pallium ; Lateral pallium ; Piriform cortex ; Entorhinal cortex ; Endopiriform nuclei ; Amygdala ; Pallial extended amygdala ; Preoptic area ; Hypothalamus
• 刊名：Journal of Chemical Neuroanatomy
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：75
• 期：part_PA
• 页码：2-19
• 全文大小：9671 K

文摘

The progeny of Dbx1-expressing progenitors was studied in the developing mouse pallium, using two transgenic mouse lines: (1) Dbx1^nlslacZ mice, in which the gene of the β-galactosidase reporter (LacZ) is inserted directly under the control of the Dbx1 promoter, allowing short-term lineage tracing of Dbx1-derived cells; and (2) Dbx1^CRE mice crossed with a Cre-dependent reporter strain (ROSA26^{loxP-stop-loxP-LacZ}), in which the Dbx1-derived cells result permanently labeled ( Bielle et al., 2005). We thus examined in detail the derivatives of the postulated longitudinal ventral pallium (VPall) sector, which has been defined among other features by its selective ventricular zone expression of Dbx1 (the recent ascription by Puelles, 2014 of the whole olfactory cortex primordium to the VPall was tested). Earlier notions about a gradiental caudorostral reduction of Dbx1 signal were corroborated, so that virtually no signal was found at the olfactory bulb and the anterior olfactory area. The piriform cortex was increasingly labeled caudalwards. The only endopiriform grisea labeled were the ventral endopiriform nucleus and the bed nucleus of the external capsule. Anterior and basolateral parts of the whole pallial amygdala also were densely marked, in contrast to the negative posterior parts of these pallial amygdalar nuclei (leaving apart medial amygdalar parts ascribed to subpallial or extratelencephalic sources of Dbx1-derived GABAergic and non-GABAergic neurons). Alternative tentative interpretations are discussed to explain the partial labeling obtained of both olfactory and amygdaloid structures. This includes the hypothesis of an as yet undefined part of the pallium, potentially responsible for the posterior amygdala, or the hypothesis that the VPall may not be wholly characterized by Dbx1 expression (this gene not being necessary for VPall molecular distinctness and histogenetic potency), which would leave a dorsal Dbx1-negative VPall subdomain of variable size that might contribute partially to olfactory and posterior amygdalar structures.