Development of an alternative culture medium for the selective enumeration of Lactobacillus casei in fermented milk
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- 作者：Monique Colombo ; Aline Evelyn Zimmermann de Oliveira ; Antonio Fern ; es de Carvalho ; Lu铆s Augusto Nero
- 关键词：Lactobacillus casei ; Selective enumeration ; Fermented milk ; Petrifilm
- 刊名：Food Microbiology
- 出版年：May, 2014
- 年：2014
- 卷：39
- 期：Complete
- 页码：89-95
- 全文大小：351 K
文摘
Monitoring the populations of probiotic strains of the species Lactobacillus casei in food is required by food industries in order to assure that a minimum concentration of these organisms will be ingested by consumers. In this context, Petrifilm鈩?AC plates can be used along with selective culture media to allow the enumeration of specific groups of lactic acid bacteria. The present study aimed to assess chemical substances as selective agents for Lb. casei in order to propose a selective culture medium to be used with Petrifilm鈩?AC plates as an alternative protocol for the enumeration of probiotic strains of this species in fermented milk. Twenty-six probiotic and starter cultures (including six strains of Lb. casei) were plated on de Man Rogosa and Sharpe (MRS) agar with distinct concentrations of nalidixic acid, bile, lithium chloride, metronidazole, sodium propionate, and vancomycin. Vancomycin at 10聽mg/L demonstrated selective activity for Lb. casei. In addition, 2,3,5-triphenyltetrazolium chlorine was identified as a compound that did not inhibit Lb. casei, and Petrifilm鈩?AC plates used with MRS and vancomycin at 10聽mg/L (MRS-V) demonstrated more colonies of this organism when incubated under anaerobic conditions than aerobic conditions. Acidophilus milk and yoghurt were prepared, added to Lb. casei strains, and stored at 4聽掳C. Lb. casei populations were monitored using MRS-V and MRTLV by conventional plating and associated with Petrifilm鈩?AC plates. All correlation indices between counts obtained by conventional plating and Petrifilm鈩?AC were significant (p聽<聽0.05), but the best performance was observed for growth on MRS-V. The obtained data indicate the efficiency of using MRS-V associated with Petrifilm鈩?AC plates for the enumeration of Lb. casei strains in fermented milk. However, the selective potential of this culture medium must be evaluated considering the specific strains of Lb. casei and the starter cultures inoculated in the fermented milk that requires monitoring.