Signal and binding. II. Converting physico-chemical responses to macromolecule-ligand interactions into thermodynamic binding isotherms

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• 作者：Wlodzimierz Bujalowski^a ; ^b ; ^{wbujalow@utmb.edu} ; Maria J. Jezewska^a ; Paul J. Bujalowski^a
• 关键词：Physico-chemical titrations ; Binding isotherms ; Thermodynamics
• 刊名：Biophysical Chemistry
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：222
• 期：Complete
• 页码：25-40
• 全文大小：1494 K
• 卷排序：222

文摘

In general, there is no linear relationship between observed physico-chemical signals and the total average degree of binding. Signal and mass conservation relationships allow the construction of binding isotherms from physico-chemical titration curves. Ligand Binding Density Function (LBDF) Method allows the construction of binding isotherms using the ligand signal. The Empirical Function (EF) Method relates the observed signal to the total average degree of binding.