A flow-batch luminometer

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• 作者：Renato A.N. Andrade^a ; Sté ; fani I.E. Andrade^a ; Valdomiro L. Martins^b ; Pablo N.T. Moreira^a ; Daniel J.E. Costa^a ; Wellington S. Lyra^a ; Má ; rio Cé ; sar U. Araú ; jo^a ; ^{laqa@quimica.ufpb.br}
• 关键词：Flow&ndash ; batch luminometer ; Chemiluminescence ; Injection ampoules ; Vitamin B12
• 刊名：Microchemical Journal
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：108
• 期：Complete
• 页码：151-155
• 全文大小：354 K

文摘

The traditional flow chemiluminescence (CL) systems typically use flow cells in spiral shape mounted in front of a photomultiplier tube (PMT). The sample is transported by using carrier fluids, undergoes dispersion, and mixes with reagents and CL reaction occurs before reaching the flow cell. As a consequence, the maximum of the chemiluminescence intensity is not exploited and the sensitivity is significantly diminished. In this work, these drawbacks were overcome by using a flow-batch approach and a large area silicon photodiode instead of PMT in order to build a simple and automatic luminometer for CL measurements. The feasibility of the proposed flow-batch luminometer was demonstrated in the determination of vitamin B₁₂ in injection ampoules, yielding limits of detection and quantification (0.11 and 0.36 ¦Ìg L^? 1, respectively), recovery rates (between 97.8 and 102.1 % ), relative standard deviations (RSD < 2.2 % , n = 3) and sample throughput (about 72 h^? 1). Thus, it was possible to both project and build a simple, flexible, versatile and automatic luminometer, while keeping the excellent characteristics of the previous flow-batch analyzer such as: low reagent and sample consumption and minimal waste generation.