A new vaccine adjuvant (BOS 2000) a potent enhancer mixed Th1/Th2 immune responses in mice immunized with HBsAg
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- 作者：Anamika Khajuria ; Amit Gupta ; Fayaz Malik ; Surjeet Singh ; Jaswant Singh ; B.D. Gupta ; K.A. Suri ; Pankaj Suden ; V.K. Srinivas ; Krishna Ella ; et al.
- 关键词：Boswellia serrata ; Hepatitis B (HBsAg) surface antigen ; Humoral and cellular
- 刊名：Vaccine
- 出版年：2007
- 出版时间：6 June 2007
- 年：2007
- 卷：25
- 期：23
- 页码：4586-4594
- 全文大小：718 K
文摘
Adjuvants in vaccines are immune stimulants that play an important role in the induction of effective and appropriate immune responses to vaccine component. In search of a potent vaccine adjuvant, the water-soluble biopolymeric fraction BOS 2000 from Boswellia serrata was evaluated for desired activity. We investigated the ability of BOS 2000 to enhance HBsAg specific immune responses. The effect was determined in the form of protective anti-HBsAg titers, neutralizing antibodies (IgG1 and IgG2a), spleen cell lymphocyte proliferation by using MTT assay, Th1 (IFN-γ and TNF-α) and Th2 (IL-4) cytokines as well as T-lymphocyte subsets (CD4/CD8) and intracellular cytokines (IFN-γ/IL-4), these responses were highest in BOS 2000 immunized mice. Alum induced only a modest enhancement of antibody responses. Reducing the dose of adjuvant by 18.1-fold in comparison to alum, total IgG and its subtypes (IgG1 and IgG2a) antibodies titer in serum was significantly enhanced. Analysis of HBsAg specific cytokines revealed that alum was associated with a predominantly IL-4 response. In contrast, BOS 2000 was associated with production of both IFN-γ and IL-4. We conclude that BOS 2000 is a potent enhancer of antigen-specific Th1 and Th2 immune responses in comparison to alum with Th2 limitation and is a promising adjuvant for vaccine applications.