The molybdenum cofactor biosynthesis complex interacts with actin filaments via molybdenum insertase Cnx1 as anchor protein in Arabidopsis thaliana
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- 作者：David Kaufholdta ; d.kaufholdt@tu-bs.de" class="auth_mail" title="E-mail the corresponding author ; Christin-Kirsty Bailliea ; c.baillie@tu-bs.de" class="auth_mail" title="E-mail the corresponding author ; Rolf Bikkera ; bikker.rolf@mh-hannover.de" class="auth_mail" title="E-mail the corresponding author ; Valentin Burkarta ; v.burkart@tu-bs.de" class="auth_mail" title="E-mail the corresponding author ; Christian-Alexander Dudeka ; c.dudek@tu-bs.de" class="auth_mail" title="E-mail the corresponding author ; Linn von Peina ; l.von-pein@tu-bs.de" class="auth_mail" title="E-mail the corresponding author ; Martin Rothkegelb ; m.rothkegel@tu-bs.de" class="auth_mail" title="E-mail the corresponding author ; Ralf R. Mendela ; r.mendel@tu-bs.de" class="auth_mail" title="E-mail the corresponding author ; Robert Hä ; nscha ; r.haensch@tu-bs.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：ABD2 ; actin binding domain 2 ; AO ; aldehyde oxidase ; BiFC ; bimolecular fluorescence complementation ; CKL6 ; casein-kinase-1-like ; CLuc ; C-terminus of the luciferase ; Cnx ; cofactor for nitrate reductase and xanthine dehydrogenase ; cPMP ; cyclic pyranopterin monophosphate ; CytD ; Cytochalasin D ; eqFP611 ; Entacmaea quadricolor fluorescence protein ; F-Actin ; filamentous actin ; GFP ; green fluorescence protein ; LA ; Lifeact ; LatB ; Latrunculin B ; MAP65 ; Microtubule Associated Protein 65-1 ; mARC ; mitochondri
- 刊名：Plant Science
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：244
- 期：Complete
- 页码：8-18
- 全文大小：3224 K
文摘
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Spatial micro-compartmentation of Mo-cofactor biosynthesis complex is observed.
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BiFC is used for interaction studies in the natural environment of cells.
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Non-invasive labelling of cytoskeleton was performed via specific binding proteins.
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Molybdenum insertase Cnx1 interacts with actin filaments but not with microtubules.
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Cnx1 acts as bridge protein anchoring the biosynthesis complex on actin filaments.