High resolution scanning electron microscopy of the human organ of Corti.: A study using freshly fixed surgical specimens
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- 作者：Glueckert ; Rudolf ; Pfaller ; Kristian ; Kinnefors ; Anders ; Schrott-Fischer ; Anneliese ; Rask-Andersen ; Helge
- 关键词：CC ; Claudius cell ; HC ; Hensen cell ; IHC ; inner hair cell ; OHC ; outer hair cell ; SEM ; scanning electron microscopy ; SGC ; spiral ganglion cell ; TCF ; tunnel crossing fibres ; TM ; tectorial membrane ; TEM ; transmission electron microscopy/microscopic ; TSB ; tunn
- 刊名：Hearing Research
- 出版年：2005
- 出版时间：January, 2005
- 年：2005
- 卷：199
- 期：1-2
- 页码：40-56
- 全文大小：1.80 M
文摘
Scanning electron microscopy on immediately fixed human cochleae obtained during surgery for life-threatening petro-clival meningioma showed excellently preserved morphology. We compared the morphological findings with those from transmission electron microscopic sections of well preserved human and animal tissue. The characteristics of neural innervation, the pathways of the nerves through the organ of Corti and the intimate relation of nerves to supporting cells along their route could be studied in detail. The lateral membranes of Hensen and Claudius cells were folded creating a surface enlargement. Marginal pillars extended the distal end of the tectorial membrane and correspond to the marginal net or aaarandfasernetzaaa described earlier. Stereocilia imprints at the undersurface of the tectorial membrane go as far as to the distal end of the marginal pillars. The presence of an irregularly distributed fourth row of outer hair cell, attached to the marginal pillars, raises questions about differences in the excitation of the last row of outer hair cells. The complex nature of many supporting cells, stria vascularis and Reissneraaas membrane, intracellular complexities as well as surface features are described. Supernumerary inner hair cells were observed and the different arrangement of outer spiral fibres in contrast to findings in animals and variations of nerve fibres within the organ of Corti between apex and base are discussed.