Studies on solvatochromic properties of aminophenylstyryl-quinolinum dye, LDS 798, and its application in studying submicron lipid based structure

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• 作者：Pabak Sarkar ; Rafal Luchowski ; Sangram Raut ; Nirupama Sabnis ; Alan Remaley ; Andras G. Lacko ; Sanjay Thamake ; Zygmunt Gryczynski ; Ignacy Gryczynski
• 关键词：Aminostyryl quinolinum dye ; Fluorescence correlation Spectroscopy ; Solvatochromism
• 刊名：Biophysical Chemistry
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：153
• 期：1
• 页码：61-69
• 全文大小：1082 K

文摘

The styryl group of dyes has been used in cellular studies for over 20 years because of their solvatochromic and/or electrochromic properties. Here we report characterization of solubility and solvatochromic properties of a near infra-red styryl dye, styryl 11 or LDS 798. We have extended our studies to small unilamellar vesicles and lipid based nanoparticles and found that solvatochromic properties of this dye used in tandem with fluorescence correlation spectroscopy can be used to efficiently determine the diffusion coefficient and hence the size of the submicron lipid based particles. This technique has the potential to provide essential information about liposomal and vesicular structures and their movement in vitro and in situ.