Evaluation of antioxidant and antimicrobial activities of the phenolic composition of Algerian Arbutus unedo L. roots

详细信息    查看全文

• 作者：Nassim Djabou ; Mohamed El Amine Dib ; Hocine Allali ; Andreas Benderb ; Mohammad A. Kamal ; Said Ghalem ; Boufeldja Tabti
• 关键词：Arbutus unedo L. ; Roots ; Catechin ; Antioxidant and antimicrobial activity ; Phenolic compounds
• 刊名：Pharmacognosy Journal
• 出版年：November-December, 2013
• 年：2013
• 卷：5
• 期：6
• 页码：275-280
• 全文大小：288 K

文摘

Introduction

The composition of phenols and flavonoids present in ethyl acetate and methanolic extracts of Algerian Arbutus unedo L. roots was in this work investigated using a combination of analytical techniques.

Methods

Firstly, the total phenolic and flavonoid content was established by an analysis of both extracts using capillary GC-MS and ¹H, ¹³C NMR spectroscopy after fractionation via column chromatography. Secondly, a screening for the antioxidant and antibacterial properties of the compounds identified was performed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, as well as ferric-ion reducing power (FRAP) assays. Subsequently, the antibacterial activity of the compounds extracted was determined via disc-diffusion against both Gram-positive and Gram-negative bacteria.

Results

It was found that the ethyl acetate extract of Algerian A. unedo L. roots was characterized by higher concentrations of phenolic acids, which also has about 2-3 times better radical scavenger properties than the reference antioxidant used, ascorbic acid. In addition, this extract showed the best antibacterial activity obtained in this study against both Gram-positive bacteria as well as Gram negative bacteria, with minimal inhibition dosage values as low as 25 Ag/disc.

Conclusion

To conclude both the antioxidant and the antibacterial properties of the extracts of Algerian A. unedo L. roots could hence be of practical value.