Comparison of CRF-immunoreactive neurons distribution in mouse and rat brains and selective induction of Fos in rat hypothalamic CRF neurons by abdominal surgery

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• 作者：Lixin Wang ; ¹ ; ^{lixinw@ucla.edu} ; Miriam Goebel-Stengel¹ ; Andreas Stengel ; S. Vincent Wu ; Gordon Ohning ; Yvette Taché
• 关键词：Corticotropin-releasing factor ; CRF antibody ; Fos ; Paraventricular nucleus of the hypothalamus ; Dorsal motor nucleus of the vagus ; Abdominal surgery ; Rat ; Mouse
• 刊名：Brain Research
• 出版年：2011
• 出版时间：30 September, 2011
• 年：2011
• 卷：1415
• 期：Complete
• 页码：34-46
• 全文大小：1K

文摘

Mice and rats are widely used in stress-related behavioral studies while little is known about the distribution of the stress hormone, corticotropin-releasing factor (CRF) in the mouse brain. We developed and characterized a novel rat/mouse CRF polyclonal antibody (CURE ab 200101) that was used to detect and compare the brain distributions of CRF immunoreactivity in na?ve and colchicine-treated rats and mice. We also assessed whether the visceral stressor of abdominal surgery activated brain CRF neurons using double labeling of Fos/CRF in na?ve rats. CRF-ir neurons were visualized in the cortex, bed nucleus of the stria terminalis, central amygdala, hypothalamic paraventricular nucleus (PVN), Barrington's nucleus and dorsolateral tegmental area in na?ve rats. CRF-immunoreactive (ir) neurons in the mouse brain were detected only after colchicine. The pattern shows fundamental similarity compared to the colchicine-treated rat brain, however, there were differences with a lesser distribution in both areas and density except in the lateral septum and external subnucleus of the lateral parabrachial nucleus which contained more CRF-ir neurons in mice, and CRF-ir neurons in the dorsal motor nucleus of the vagus were found only in mice. Abdominal surgery in na?ve rats induced Fos-ir in 30 % of total CRF-ir neurons in the PVN compared with control (anesthesia alone) while Fos was not co-localized with CRF in other brain nuclei. These data indicate that CRF-ir distribution in the brain displays similarity as well as distinct features in mice compared to rats that may underlie some differential stress responses. Abdominal surgery activates CRF-ir neurons selectively in the PVN of rats without colchicine treatment.