Coassembled nanostructured bioscaffold reduces the expression of proinflammatory cytokines to induce apoptosis in epithelial cancer cells

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• 作者：Rui Li ; BSc^a ; ^f ; Sivapriya Pavuluri ; PhD^a ; ^g ; Kiara Bruggeman ; BSc^b ; Benjamin M. Long ; PhD^a ; Andrew J. Parnell ; PhD^c ; Anne Martel ; PhD^d ; Steven R. Parnell ; PhD^e ; Frederick M. Pfeffer ; PhD^a ; Andrew J.C. Dennison ; PhD^d ; ⁱ ; Kevin R. Nicholas ; PhD^a ; ^g ; Colin J. Barrow ; PhD^a ; David R. Nisbet ; PhD^b ; ¹ ; Richard J. Williams ; PhD^a ; ^h ; ¹ ; ^{Richard.Williams@RMIT.edu.au" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bionanotechnology ; Self-assembly ; Cancer ; Supramolecular materials ; Hydrogels ; Tissue engineering
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：12
• 期：5
• 页码：1397-1407
• 全文大小：1931 K

文摘

The local inflammatory environment of the cell promotes the growth of epithelial cancers. Therefore, controlling inflammation locally using a material in a sustained, non-steroidal fashion can effectively kill malignant cells without significant damage to surrounding healthy cells. A promising class of materials for such applications is the nanostructured scaffolds formed by epitope presenting minimalist self-assembled peptides; these are bioactive on a cellular length scale, while presenting as an easily handled hydrogel. Here, we show that the assembly process can distribute an anti-inflammatory polysaccharide, fucoidan, localized to the nanofibers within the scaffold to create a biomaterial for cancer therapy. We show that it supports healthy cells, while inducing apoptosis in cancerous epithelial cells, as demonstrated by the significant down-regulation of gene and protein expression pathways associated with epithelial cancer progression. Our findings highlight an innovative material approach with potential applications in local epithelial cancer immunotherapy and drug delivery.