Molecular evolution of the androgenic hormone in terrestrial isopods

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• 作者：Nicolas Cerveau^a ; ¹ ; ^{nicolas.cerveau@geo.uni-goettingen.de" class="auth_mail} ; Didier Bouchon^a ; ^{didier.bouchon@univ-poitiers.fr" class="auth_mail} ; Thierry Bergè ; s^b ; ^{thierry.berges@univ-poitiers.fr" class="auth_mail} ; Pierre Grè ; ve^a ; ^{pierre.greve@univ-poitiers.fr" class="auth_mail}
• 关键词：AG ; androgenic gland ; AGH ; androgenic gland hormone ; RT-PCR ; reverse transcriptase polymerase chain reaction ; M-MLV ; Moloney murine leukemia virus ; NTP ; deoxy-ribonucleoside triphosphate ; CBS ; Center for Biological sequence analysis ; REL ; random effect likelihood ; IAG ; insulin-like androgenic gland factor ; IUB ; International Union of Biochemistry
• 刊名：Gene
• 出版年：25 April, 2014
• 年：2014
• 卷：540
• 期：1
• 页码：71-77
• 全文大小：1078 K

文摘

In crustaceans, the androgenic gland (AG), thanks to the synthesis of the androgenic gland hormone (AGH), controls the differentiation of the primary and secondary male sexual characters. In this study, we amplified 12 new AGH cDNAs in species belonging to five different families of the infra-order Ligiamorpha of terrestrial isopods. Putative essential amino acids for the production of a functional AGH protein exhibit signatures of negative selection and are strictly conserved including typical proteolytic cleavage motifs, a putative N-linked glycosylation motif on the A chains and the eight Cys positions. An insulin-like growth factor motif was also identified in Armadillidium AGH sequences. The phylogenetic relationships of AGH sequences allowed one to distinguish two main clades, corresponding to members of the Armadillidiidae and the Porcellionidae families which are congruent with the narrow specificity of AG heterospecific grafting. An in-depth understanding of the regulation of AGH expression would help deciphering the interaction between Wolbachia, widespread feminizing endosymbiotic bacteria in isopods, and the sex differentiation of their hosts.