Herb-drug interaction of Andrographis paniculata (Nees) extract and andrographolide on pharmacokinetic and pharmacodynamic of naproxen in rats

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• 作者：Aishwarya Balap^a ; Sathiyanarayanan Lohidasan^a ; Arulmozhi Sinnathambi^b ; Kakasaheb Mahadik^a ; ^{krmahadik@redifffmail.com}
• 关键词：AN ; Andrographolide ; ANOVA ; Analysis of variance ; APE ; Andrographis paniculata Nees Extract ; AUC 0-&infin ; Area under plasma concentration-time curve extrapolated to infinity ; AUC0&minus ; t ; Area under plasma concentration-time curve from zero to last time point ; AUMC0-&infin ; Area under the concentration time (c× ; t) versus time (t) curve for extrapolated to infinity ; AUMC0&minus ; t ; Area under the concentration time (c× ; t) versus time (t) curve for last time point ; CL ; Plasma clearanc
• 刊名：Journal of Ethnopharmacology
• 出版年：2017
• 出版时间：4 January 2017
• 年：2017
• 卷：195
• 期：Complete
• 页码：214-221
• 全文大小：1332 K
• 卷排序：195

文摘

Andrographis paniculata Nees (Acanthacae) have broad range of pharmacological effects such as hepatoprotective, antifertility, antimalarial, antidiabetic, suppression of various cancer cells and anti-inflammatory properties and is widely used medicinal plant in the traditional Unani and Ayurvedic medicinal systems. Andrographolide (AN) is one of the active constituent of the A. paniculata Nees extract (APE). They have been found in many traditional herbal formulations in India and proven to be effective as anti-inflammatory drug.Aim of the studyTo evaluate the pharmacokinetic and pharmacodynamic (anti arthritic) herb-drug interactions of A. paniculata Nees extract (APE) and pure andrographolide (AN) with naproxen (NP) after oral co-administration in wistar rats.Materials and methodsAfter oral co-administration of APE (200 mg/Kg) and AN (60 mg/kg) with NP (7.5 mg/kg) in rats, drug concentrations in plasma were determined using HPLC method. The main pharmacokinetic parameters of Cmax, tmax, t1/2, MRT, Vd, CL, and AUC were calculated by non-compartment model. Change in paw volume, mechanical nociceptive threshold, mechanical hyperalgesia, histopathology and hematological parameters were evaluated to study antiarthritic activity.ResultsCo-administration of NP with APE and pure AN decreased systemic exposure level of NP in vivo. The Cmax, tmax, AUC0−t of NP was decreased. In pharmacodynamic study, NP (10 mg/kg) alone and NP+AN (10+60 mg/kg) groups exhibited significant synergistic anti-arthritic activity as compared to groups NP+APE, APE and AN alone.ConclusionThe results obtained from this study suggested that NP, APE and pure AN existed pharmacokinetic herb–drug interactions in rat which is correlated with anti-arthritic study. The knowledge regarding possible herb–drug interaction of NP might be helpful for physicians as well as patients using AP. So further studies should be done to understand the effect of other herbal ingredients of APE on NP as well as to predict the herb–drug interaction in humans.