High-performance liquid chromatographic method for the determination and pharmacokinetic study of oxypeucedanin hydrate and byak-angelicin after oral administration of Angelica dahurica extracts in mongrel dog plasma
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- 作者：Ying Xie ; Yi Chen ; Mei Lin ; Jun Wen ; Guorong Fan ; Yutian Wu
- 关键词：Angelica dahurica ; Oxypeucedanin hydrate ; Byak-angelicin ; HPLC ; Pharmacokinetic study
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2007
- 出版时间：9 May 2007
- 年：2007
- 卷：44
- 期：1
- 页码：166-172
- 全文大小：178 K
文摘
A high-performance liquid chromatographic method was developed and validated for the determination and pharmacokinetic study of oxypeucedanin hydrate and byak-angelicin after oral administration of Angelica dahurica extracts in mongrel dog plasma. The coumarin components and the internal standard isopsoralen were extracted from plasma samples with the mixture of tert-butyl methyl ether and n-hexane (4:1, v/v). Chromatographic separation was performed on a C18 column (200 mm × 4.6 mm, 5 μm) with the mobile phase acetonitrile–methanol–water–acetic acid (20:15:65:2, v/v/v/v) at a flow-rate of 1.0 ml/min. Only the peak of oxypeucedanin hydrate and byak-angelicin could be detected in dog plasma after oral administration of ethanol extracts of A. dahurica mainly containing xanthotoxol, osthenol, imperatorin, oxypeucedanin hydrate and byak-angelicin. The calibration curves of oxypeucedanin hydrate and byak-angelicin were linear over a range of 22.08–8830.00 and 6.08–2430.00 ng/ml in dog plasma, respectively. The quantification limit of oxypeucedanin hydrate and byak-angelicin in dog plasma was 22.08 and 6.08 ng/ml, respectively. The intra- and inter-day precision was less than 7.6 % and 8.5 % and the accuracy was from 91.9 % to 106.1 % . The lowest absolute recoveries of oxypeucedanin hydrate and byak-angelicin were 85.7 % and 87.0 % , respectively. The method was successfully applied to the pharmacokinetic studies of oxypeucedanin hydrate and byak-angelicin in dog plasma after oral administration of ethanol extracts from A. dahurica.