Angelicin regulates LPS-induced inflammation via inhibiting MAPK/NF-魏B pathways

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• 作者：Fang Liu ; Guo-quan Sun ; Hua-yi Gao ; Rui-sheng Li ; Lanan-Wassy Soromou ; Na Chen ; Yan-Hong Deng ; Hai-hua Feng
• 关键词：Angelicin ; Lipopolysaccharide ; Cytokines ; NF-魏B ; MAPK
• 刊名：Journal of Surgical Research
• 出版年：November, 2013
• 年：2013
• 卷：185
• 期：1
• 页码：300-309
• 全文大小：2174 K

文摘

Background

Angelicin is a furocoumarin found in Psoralea corylifolia L. fruit. The purpose of this study was to investigate the protective ability of angelicin against inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and LPS-induced in聽vivo acute lung injury model.

Materials and methods

The concentrations of tumor necrosis factor alpha (TNF-伪) and interleukin (IL)-6 in the culture supernatants of RAW 264.7 cells were determined 24 h after LPS administration. ALI was induced by intratracheal instillation of LPS. Six hours after LPS inhalation, bronchoalveolar lavage fluid and lung tissue samples were obtained for enzyme-linked immunosorbent assay, histologic, and Western blotting analyses.

Results

The results showed that pretreatment with angelicin markedly downregulated TNF-伪 and IL-6 levels in聽vitro and in聽vivo, and significantly decreased the amount of inflammatory cells, lung wet-to-dry weight ratio, and myeloperoxidase activity in LPS-induced ALI mice. Furthermore, Western blotting analysis results demonstrated that angelicin blocked the phosphorylation of I魏B伪, NF-魏Bp65, p38 MAPK, and JNK in LPS-induced ALI.

Conclusions

These results suggest that angelicin was potentially advantageous to prevent inflammatory diseases by inhibiting NF-魏B and MAPK pathways. Our data indicated that angelicin might be a potential new agent for prevention of inflammatory reactions and diseases in the clinic.