Molecular cloning and characterization of a novel angiopoietin family protein, angiopoietin-3
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- 作者：Kim ; Injune ; Kwak ; Hee Jin ; Ahn ; Ji Eun ; So ; June-No ; Liu ; Mingzhu ; Koh ; Keum Nim ; Koh ; Gou Young
- 关键词：Angiopoietin ; Endothelial cell ; Vasculogenesis ; PCR ; polymerase chain reaction ; CMV ; cytomegalovirus ; cDNA ; complementary deoxyribonucleic acid
- 刊名：FEBS Letters
- 出版年：1999
- 出版时间：January 29, 1999
- 年：1999
- 卷：443
- 期：3
- 页码：353-356
- 全文大小：318 K
文摘
Using homology-based PCR, we have isolated cDNA encoding a novel member (491 amino acids) of the angiopoietin (Ang) family from human adult heart cDNA and have designated it angiopoietin-3 (Ang3). The NH2-terminal and COOH-terminal portions of Ang-3 contain the characteristic coiled-coil domain and fibrinogen-like domain that are conserved in other known Angs. Ang3 has a highly hydrophobic region at the N-terminus (
21 amino acids) that is typical of a signal sequence for protein secretion. Ang3 mRNA is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine in human adult tissues. Additionally, Ang3 is a secretory protein, but is not a mitogen in endothelial cells.
21 amino acids) that is typical of a signal sequence for protein secretion. Ang3 mRNA is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine in human adult tissues. Additionally, Ang3 is a secretory protein, but is not a mitogen in endothelial cells.