A SPECT–CT system (VECTor/CT) with stationary gamma-detectors was equipped with a tungsten collimator with clustered pinholes. Images were reconstructed with pixel-based OSEM, using a dedicated 131I system matrix that models the distance- and energy-dependent resolution and sensitivity of each pinhole, as well as the intrinsic detector blurring and variable depth of interaction in the detector. The system performance was characterized with phantoms and in vivo static and dynamic 131I-NaI scans of mice.
Reconstructed image resolution reached 0.6 mm, while quantitative accuracy measured with a 131I filled syringe reaches an accuracy of + 3.6 ± 3.5% of the gold standard value. In vivo mice scans illustrated a clear shape of the thyroid and biodistribution of 131I within the animal. Pharmacokinetics of 131I was assessed with 15-s time frames from the sequence of dynamic images and time–activity curves of 131I-NaI.
High-resolution quantitative and fast dynamic 131I SPECT in mice is possible by means of a high-energy collimator and optimized system modeling. This enables analysis of 131I uptake even within small organs in mice, which can be highly valuable for development and optimization of targeted cancer therapies.