Antarctic teleost immunoglobulins: More extreme, more interesting

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• 作者：Maria Rosaria Coscia  ; ;   ; ^{mr.coscia@ibp.cnr.it} ; Sonia Varriale ;   ; ^{s.varriale@ibp.cnr.it} ; Stefano Giacomelli ;   ; ^{s.giacomelli@ibp.cnr.it} ; Umberto Oreste ;   ; ^{u.oreste@ibp.cnr.it}
• 关键词：Antibody response to parasites ; Extracellular membrane proximal domain ; Alternative mRNA splicing ; Antarctic teleost ; VH gene family
• 刊名：Fish & Shellfish Immunology
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：31
• 期：5
• 页码：688-696
• 全文大小：1859 K

文摘

We have investigated the immunoglobulin molecule and the genes encoding it in teleosts living in the Antarctic seas at the constant temperature of ?.86?¡ãC. The majority of Antarctic teleosts belong to the suborder Notothenioidei (Perciformes), which includes only a few non-Antarctic species. Twenty-one Antarctic and two non-Antarctic Notothenioid species were included in our studies. We sequenced immunoglobulin light chains in two species and ¦Ì heavy chains, partially or totally, in twenty species. In the case of heavy chain, genomic DNA and the cDNA encoding the secreted and the membrane form were analyzed. From one species, Trematomus bernacchii, a spleen cDNA library was constructed to evaluate the diversity of VH gene segments. T. bernacchii IgM, purified from the serum and bile, was characterized. Homology Modelling and Molecular Dynamics were used to determine the molecular structure of T.?bernacchii and Chionodraco hamatus immunoglobulin domains. This paper sums up the previous results and broadens them with the addition of unpublished data.