Production of recombinant lipase B from Candida antarctica in Pichia pastoris under control of the promoter PGK using crude glycerol from biodiesel production as carbon source

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• 作者：Julia Macedo Robert^a ; Fernanda Senna Lattari^a ; ^c ; Antonio Carlos Machado^a ; Aline Machado de Castro^b ; Rodrigo Volcan Almeida^c ; Fernando Araripe Gonç ; alves Torres^d ; Francisco Valero^e ; ^{Francisco.Valero@uab.cat} ; Denise Maria Guimarã ; es Freire^a ; ^{freire@iq.ufrj.br}
• 关键词：PGK promoter ; Heterologous Candida antarctica ; Lipase ; Crude glycerol
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：118
• 期：Complete
• 页码：123-131
• 全文大小：1039 K
• 卷排序：118

文摘

Use of constitutive promoter (PGK) to produce rLipB from Candida antarctica. Use of crude glycerol to produce recombinant rLipB on Pichia pastoris. Use of low cost culture medium. Characterization of the lipase produced and comparison between the produced one and the commercial.