Cancer cell specific cytotoxic effect of Rhoeo discolor extracts and solvent fractions

详细信息    查看全文

• 作者：Rebeca Garcí ; a-Varela^a ; ^c ; ¹ ; Oscar Raú ; l Fajardo Ramí ; rez^b ; Sergio O. Serna-Saldivar^a ; ^d ; Julio Altamirano^d ; Guy A. Cardineau^a ; ^c ; ^{Guy.Cardineau@itesm.mx" class="auth_mail" title="E-mail the corresponding author}
• 关键词：DMSO ; dimethylsulfoxide ; MTS ; 3-(4 ; 5-dimethyl thiazole-2-yl)-2 ; 5-diphenyl tetrazolium bromide ; MeOH ; Methanol ; EtOH ; Ethanol ; EtAc ; Ethyl Acetate ; Ac ; Acetone ; PtEt ; Petroleum Ether ; CHCl3 ; Chloroform ; Hx ; Hexane ; PI ; Propidium Iodine
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：22 August 2016
• 年：2016
• 卷：190
• 期：Complete
• 页码：46-58
• 全文大小：1840 K

文摘

Traditional or folk medicine has led to the discovery of important bioactive substances used in several health-related areas. Phytochemicals in Rhoeo discolor (R. discolor) extracts have proven to have important cancer cell specific cytotoxic activity. In the present research, we determined the cytotoxic effect of extracts of R. discolor, a plant commonly used in Mexico for both medicinal and ornamental purposes.

Aim of the study

We evaluated the cytotoxic effects against three representative human cancer cell lines: HT-29 colon cancer, Hep-G2 liver cancer and PC-3 prostate cancer cell lines, as well as a control fibroblast cell line NIH 3T3.

Materials and methods

Ten different crude extracts were tested along with fractions derived from the five most bioactive crude extracts. Analytical data, HPLC-MS-TOF, revealed a high content of phenolic compounds such as anthocyanins, ferulic, vanillic, chlorogenic and p-coumaric acid in the extracts. Phenolic compounds have previously been reported as health beneficial with antioxidant and potential cancer specific cytotoxic effects.

Results

Studies revealed that low concentrations of these crude bioactive extracts (10 µg/ml) and their fractions (50 µg/ml) were effective as cancer specific cytotoxic agents, since they caused a significant proliferation inhibition on cancer cell lines (up to 94.2% in HT-29, 92.9% in Hep-G2 and 61.8% in PC-3 of apoptosis induction) with little harm to the control cell line (no higher than 28.3% apoptosis induction), and, importantly, the most effective extracts were mainly water, methanol and ethanol based.

Conclusions

These results suggest that a diet containing these compounds may function as a medical aid or chemoprotective.