The role of grancalcin in adhesion of neutrophils
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- 作者：Ping Xu ; Jü ; rgen Roes ; Anthony W. Segal and Marko Radulovic
- 关键词：Neutrophil ; Grancalcin ; Adhesion ; Focal adhesion complex ; Phagocytosis ; Granule
- 刊名：Cellular Immunology
- 出版年：2006
- 出版时间：April 2006
- 年：2006
- 卷：240
- 期：2
- 页码：116-121
- 全文大小：777 K
文摘
Grancalcin is a protein specifically expressed in neutrophils and monocytes/macrophages. The function of grancalcin has not been identified. Grancalcin-deficient neutrophils were previously demonstrated to exert normal recruitment to the inflamed site, NADPH oxidase activation, extracellular release of secondary granules, apoptosis and activation-induced Ca2+ flux. In this study we analyzed granule numbers in resting and activated grancalcin-deficient neutrophils, their phagocytic activity and adherence to extracellular matrix proteins. Results revealed normal phagocytosis and degranulation of grancalcin-deficient neutrophils, while their adhesion to fibronectin was decreased by 60 % . Consistently, the processes associated with neutrophil adhesion, such as formation of focal adhesion complexes and spreading, were also impaired in grancalcin-deficient neutrophils by 89 and 38 % , respectively. In contrast, adherence to other extracellular matrix proteins: collagen, laminin and vitronectin, was not significantly altered. We thus report for the first time a function of grancalcin.