Proinflammatory effects and oxidative stress within human bronchial epithelial cells exposed to atmospheric particulate matter (PM_2.5 and PM_>2.5) collected from Cotonou, Benin

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• 作者：Boris Fresnel Cachon ; St茅phane Firmin ; Anthony Verdin ; Lucie Ayi-Fanou ; Sylvain Billet ; Fabrice Cazier ; Perrine J. Martin ; Faustin Aissi ; Dominique Courcot ; Ambaliou Sanni ; Pirouz Shirali
• 关键词：Benin ; Cytotoxicity ; Inflammatory response ; Oxidative stress ; Particulate matter
• 刊名：Environmental Pollution
• 出版年：February, 2014
• 年：2014
• 卷：185
• 期：Complete
• 页码：340-351
• 全文大小：2392 K

文摘

After particulate matter (PM) collection in Cotonou (Benin), a complete physicochemical characterization of PM_2.5 and PM_>2.5 was led. Then, their adverse health effects were evaluated by using in聽vitro culture of human lung cells. BEAS-2B (bronchial epithelial cells) were intoxicated during short-term exposure at increasing PM concentrations (1.5-96聽渭g/cm²) to determine global cytotoxicity. Hence, cells were exposed to 3 and 12聽渭g/cm² to investigate the potential biological imbalance generated by PM toxicity. Our findings showed the ability of both PM to induce oxidative stress and to cause inflammatory cytokines/chemokines gene expression and secretion. Furthermore, PM were able to induce gene expression of enzymes involved in the xenobiotic metabolism pathway. Strong correlations between gene expression of metabolizing enzymes, proinflammatory responses and cell cycle alteration were found, as well as between proinflammatory responses and cell viability. Stress oxidant parameters were highly correlated with expression and protein secretion of inflammatory mediators.