A novel antibody targeting CD24 and hepatocellular carcinoma in vivo by near-infrared fluorescence imaging

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• 作者：Hua He¹ ; Xiaojie Tu¹ ; Juan Zhang ; ^{juancpu@126.com" class="auth_mail" title="E-mail the corresponding author} ; Desmond Omane Acheampong ; Li Ding ; Zhaoxiong Ma ; Xueyan Ren ; Chen Luo ; Zhiguo Chen ; Tong Wang ; Wei Xie ; Min Wang ; ^{minwang@cpu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ELISA ; enzyme-linked immunosorbent assay ; FACS ; fluorescence activated cell sorter ; FITC ; fluorescein isothiocyanate ; FBS ; fetal bovine serum ; HRP ; horeradishperoxidase ; IgG ; immunoglobulin G ; mAb ; monoclonal antibody ; scFv ; single-chain antibody fragment
• 刊名：Immunobiology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：220
• 期：12
• 页码：1328-1336
• 全文大小：2105 K

文摘

Liver cancer is one of the most common malignant cancers worldwide. The poor response of liver cancer to chemotherapy has whipped up the interest in targeted therapy with monoclonal antibodies because of its potential efficiency. One promising target is cluster of differentiation 24 (CD24), which is known to beover-expressed on hepatocellular carcinoma (HCC), providing prospect for HCC targeted diagnosis and therapy. In this study we developed a novel CD24 targeted monoclonal antibody G7mAb based on hybridoma technology and then generated a single-chain antibodyfragment (scFv) G7S. Firstly, ELISA, western blot, and flow cytometry assays demonstrated specific binding of CD24 by G7mAb and G7S. Further, G7mAb was demonstrated to have similar binding capacity as ML5 (a commercial Anti-CD24 Mouse Antibody) inimmunohistochemical assay. Further more, a near-infrared fluorescent dye multiplex probe amplification (MPA) was conjugated to G7mAb and G7S to form G7mAb-MPA and G7S-MPA. The near-infrared 铿倁orescence imaging revealed that G7mAb and G7S aggregate in CD24 + Huh7 hepatocellular carcinoma xenograft tissuevia specific binding to CD24 in vivo. In conclussion, G7mAb and G7S were tumor targeted therapeutic and diagnostic potentials in vitro and in vivo as anticipated.