Development of single chain variable fragment (scFv) antibodies against surface proteins of ‿span style='font-style: italic'>Ca. Liberibacter asiaticus‿
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- 作者：Qing Yuana ; 1 ; Ramon Jordanb ; Ronald H. Brlanskyc ; Olga Minenkovad ; John Hartunga ;
- 关键词：Huanglongbing ; HLB ; Citrus greening ; Phage display ; Phagemid ; scFv
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：122
- 期：Complete
- 页码：1-7
- 全文大小：1019 K
文摘
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‘Ca. Liberibacter asiaticus’ causes HLB, a devastating disease of citrus.
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Psyllids with high concentrations of ‘Ca. L. asiaticus’ were used to immunize mice.
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A scFv antibody library was made from mRNA isolated from spleens of immunized mice.
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Cloned surface antigens from ‘Ca. L. asiaticus’ were used to select scFv.
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Several scFv detected ‘Ca. L. asiaticus’ in plant extracts in a dot blot format.