Established an effective in vitro refolding method for single domain antibody inclusion bodies.
Dilution refolding conditions for sdAb specific against human beta-2-microglobulin were optimized.
The antigen-binding activity of refolded sdAb was assayed by ELISA and isothermal titration calorimetry.
Provide an important strategy for the recombinant production and application of sdAb.