Anti-inflammatory properties of an isoxazole derivative - MZO-2

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• 作者：Marcin Mączyński^a ; ^{marcin.maczynski@umed.wroc.pl" class="auth_mail" title="E-mail the corresponding author} ; Jolanta Artym^b ; Maja Kocięba^b ; Iwona Kochanowska^b ; Stanisław Ryng^a ; Michał Zimecki^b
• 关键词：AFC ; antibody forming cells ; cFa ; complete Freund's adjuvant ; COX ; cyclooxygenase ; CsA ; cyclosporine ; Dex ; dexamethasone ; DMSO ; dimethylsulfoxide ; FCS ; fetal calf serum ; iFa ; incomplete Freund's adjuvant ; LPS ; lipopolysaccharide ; MTT ; (3-[4 ; 5-dimethylthiazol-2-yl]-2 ; 5-diphenyltetrazolium bromide) ; OVA ; ovalbumin ; PHA ; phytohemagglutinin ; PBMC ; peripheral blood mononuclear cells ; TNF α ; tumor necrosis factor alpha ; SRBC ; sheep red blood cells
• 刊名：Pharmacological Reports
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：68
• 期：5
• 页码：894-902
• 全文大小：1325 K

文摘

A series of new isoxazole derivatives of expected immunosuppressive activities was synthesized. Following in vitro screening in the human cell models, the activity of MZO-2 compound (ethyl N-{4-[(2,4-dimethoxybenzyl)carbamoyl]-3-methylisoxazol-5-yl}acetimidate) in mouse in vivo models was evaluated.

Methods

In vitro tests included evaluation of: peripheral blood mononuclear cells (PBMC) viability, phytohemagglutinin (PHA)-induced PBMC proliferation and lipopolysaccharide (LPS)-induced tumor necrosis factor α (TNF α) production in whole blood cell cultures. MZO-2 was studied in mice for its effects on: humoral immune response to sheep erythrocytes (SRBC), delayed type hypersensitivity (DTH) to ovalbumin (OVA), contact sensitivity to oxazolone and carrageenan-induced foot pad edema. In addition, the effect of MZO-2 on expression of caspases in Jurkat cells was determined.

Results

The studied compounds exhibited differential, dose-dependent effects to suppress PHA-induced PBMC proliferation and a weak property to suppress LPS-induced production of TNF α. MZO-2 had no effect on the induction phase of the humoral immune response to SRBC in vitro and in vivo, but moderately suppressed the induction phase of DTH to OVA. Its inhibitory effect on carrageenan-induced paw inflammation was potent. Likewise, MZO-2, applied in ointment, was very effective in reducing ear edema and number of lymphocytes in draining lymph nodes of mice sensitized to oxazolone, comparably to tacrolimus, the reference drug. The expression of caspases 3, 8 and 9 in Jurkat cells was inhibited by the compound.

Conclusion

MZO-2, applied systemically or locally, may serve as a potential drug for amelioration of inflammatory processes.