Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac₂SGL

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• 作者：Frank Camacho^a ; Marí ; a E. Sarmiento^b ; ^c ; Fatima Reyes^a ; Louise Kim^d ; Jim Huggett^d ; Marco Lepore^e ; Oscar Otero^f ; Martine Gilleron^g ; ^h ; Germain Puzo^g ; ^h ; Mohd Nor Norazmi^b ; ^c ; ⁱ ; Graham Rook^d ; Lucia Mori^e ; Gennaro De Libero^e ; Armando Acosta^c ; ⁱ ; ^{aracosta2001@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Antibodies ; CD1b ; Lipids ; Tuberculosis
• 刊名：International Journal of Mycobacteriology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：5
• 期：2
• 页码：120-127
• 全文大小：763 K

文摘

The development of new tools capable of targeting Mycobacterium tuberculosis (Mtb)-infected cells have potential applications in diagnosis, treatment, and prevention of tuberculosis. In Mtb-infected cells, CD1b molecules present Mtb lipids to the immune system (Mtb lipid–CD1b complexes). Because of the lack of CD1b polymorphism, specific Mtb lipid–CD1b complexes could be considered as universal Mtb infection markers. 2-Stearoyl-3-hydroxyphthioceranoyl-2′-sulfate-α-α′-d-trehalose (Ac₂SGL) is specific for Mtb, and is not present in other mycobacterial species. The CD1b–Ac₂SGL complexes are expressed on the surface of human cells infected with Mtb. The aim of this study was to generate ligands capable of binding these CD1b–Ac₂SGL complexes.

Methods

A synthetic human scFv phage antibody library was used to select phage-displayed antibody fragments that recognized CD1b–Ac₂SGL using CD1b-transfected THP-1 cells loaded with Ac₂SGL.

Results

One clone, D11—a single, light-variable domain (kappa) antibody (dAbκ11)—showed high relative binding to the Ac₂SGL–CD1b complex.

Conclusion

A ligand recognizing the Ac₂SGL–CD1b complex was obtained, which is a potential candidate to be further tested for diagnostic and therapeutic applications.