Highly specific and potently activating Vγ9Vδ2-T cell specific nanobodies for diagnostic and therapeutic applications
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- 作者：René ; e C.G. de Bruina ; rc.debruin@vumc.nl" class="auth_mail" title="E-mail the corresponding author ; Siné ; ad M. Lougheeda ; sm.lougheed@vumc.nl" class="auth_mail" title="E-mail the corresponding author ; Liza van der Kruka ; kruk_lizz@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Anita G. Stama ; agm.stam@vumc.nl" class="auth_mail" title="E-mail the corresponding author ; Erik Hooijbergb ; erik.hooijberg@vumc.nl" class="auth_mail" title="E-mail the corresponding author ; Rob C. Rooversc ; r.roovers@merus.nl" class="auth_mail" title="E-mail the corresponding author ; Paul M.P. van Bergen en Henegouwenc ; p.vanbergen@uu.nl" class="auth_mail" title="E-mail the corresponding author ; Henk M.W. Verheula ; h.verheul@vumc.nl" class="auth_mail" title="E-mail the corresponding author ; Tanja D. de Gruijla ; td.degruijl@vumc.nl" class="auth_mail" title="E-mail the corresponding author ; Hans J. van der Vlieta ; jj.vandervliet@vumc.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：Nanobody ; Single-domain antibody fragment ; VHH ; Gamma delta T cells ; Flow cytometry ; MACS ; Immunocytochemistry ; Immunotherapy ; Cancer
- 刊名：Clinical Immunology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：169
- 期：Complete
- 页码：128-138
- 全文大小：1208 K
文摘
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Novel Vγ9Vδ2-T cell specific VHH are generated and functionally characterized.
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Both activating and non-activating VHH have been identified.
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The VHHs are useful for FACS, MACS and immunocytochemistry to study Vγ9Vδ2-T cells.
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These VHHs are possibly of value for immunotherapeutic development.