Application of streptavidin mass spectrometric immunoassay tips for immunoaffinity based antibody phage display panning
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- 作者：Chai Fung China ; Lian Wee Lerb ; Yee Siew Choonga ; Eugene Boon Beng Onga ; Asma Ismaila ; Gee Jun Tyea ; Theam Soon Lima ; theamsoon@usm.my" class="auth_mail" title="E-mail the corresponding author
- 关键词：dAb ; domain antibody ; D.A.R.T's ; Disposable Automation Research Tips ; HlyE ; Hemolysin E ; MS ; mass spectrophotometry ; MSIA ; mass spectrometry immunoassay ; scFv ; single-chain variable fragment ; Ub ; ubiquitin
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：120
- 期：Complete
- 页码：6-14
- 全文大小：1065 K
文摘
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Application of streptavidin MSIA™ D.A.R.T's® for antibody phage display is investigated.
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A novel semi-automated affinity selection was carried out using MSIA™ Streptavidin D.A.R.T's® platform.
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Polyclonal enrichment and monoclonal domain antibodies were identified for Hemolysin E antigen.
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The proposed method provides an alternative to conventional antibody panning strategies.