An innovative detecting way of Escherichia coli O157H:H7 by a micro-fluxgate-based bio-sensing system

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• 作者：Xue-cheng Sun ^{sunxc_2007@126.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Zhen YangAuthor Vitae ; Chong Lei ; ^{Leiqhd@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Yan LiuAuthor Vitae ; Lei GuoAuthor Vitae ; Yong Zhou ; ^{yzhou@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Escherichia coli O157H ; H7 ; Immune reaction ; Micro fluxgate sensor ; Dynabeads ; Superparamagnetism
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2015
• 出版时间：31 December 2015
• 年：2015
• 卷：221
• 期：Complete
• 页码：985-992
• 全文大小：2142 K

文摘

A novel bio-sensing system based on a micro-fluxgate and use of magnetic beads labels was developed for detection of Escherichia coli O157H:H7. The micro fluxgate sensor was fabricated using standard microfabrication processes. Magnetic core of the sensor was made using Fe-based amorphous ribbon core and a 3-D magnetic sensitive element was made of Cu solenoid coils. Response of the micro-fluxgate-based bio-sensing system to superparamagnetic Dynabead was first evaluated with different concentrations of Dynabeads. It was found that a concentration as low as 10 ng/ml could be detected with an external dc magnetic field ranging from 450 μT to 700 μT. Double antibody sandwich assay was employed in preparation of E. coli O157H:H7 samples. Detection of E. coli O157H:H7 antigens at different concentrations were demonstrated. A minimum detectable concentration of 100 CFU/ml was observed for this bio-sensing system. On account of the detection performance and other advantages such as miniaturization, portability and stability, this bio-sensing system is a potential candidate for rapid analysis E. coli O157:H7 and other pathogenic bacteria. It is also of considerable interest for biomedical applications employing known specific binding of target and labels.