Separable detecting of Escherichia coli O157H:H7 by a giant magneto-resistance-based bio-sensing system

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• 作者：Xuecheng Sun ^{sunlocation@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Chong Lei ; ^{Leiqhd@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Lei GuoAuthor Vitae ; Yong Zhou ; ^{yzhou@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Escherichia coli O157H ; H7 ; Immune reaction ; GMR ; Dynabeads ; Superparamagnetism
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：29 October 2016
• 年：2016
• 卷：234
• 期：Complete
• 页码：485-492
• 全文大小：2323 K

文摘

Herein we report a new separable detection system combining magnetic nanoparticle labels with giant magnetoresistance (GMR) first time developed for Escherichia coli (E. coli) O157H:H7 detection. The GMR sensor fabricated employing standard microfabrication technique, while double antibody sandwich immunoassay and streptavidin–biotin binding assay were employed for immobilizing and labeling E. coli O157H:H7 on Au-film coated glass wafer. The GMR-based immunosensor detected E. coli O157H:H7 antigens at different sample concentrations with a minimum detectable limit of 100 CFU/mL, well accepted for early detection in clinical diagnosis and environmental monitoring. Compared with the recently reported GMR sensor based detection methods, this newly developed separable detection method possesses several advantages such as convenient manipulation, avoid being contaminated and damaged by chemical solution and reusability without cleaning. So it is of considerable interest towards the biomedical application based on known specific binding of target and labels.