Diagnostic Performances of Hepatitis C Virus-NS4 Antigen in Patients with Different Liver Pathologies

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• 作者：Abdelfattah M. Attallah^a ; ^{amattallah@hotmail.com} ; Mohamed M. Omran^b ; Wesam A. Nasif^c ; Mohamed F. Ghaly^d ; Abd El-Raheem R. El-Shanshoury^e ; Mohga S. Abdalla^b ; Hayat M. Sharada^b ; Khaled Farid^f ; Wageh El-Shony^e ; El-Sayed M. Moussa^a ; Ehab B. El-Domany^a ; ^b ; Eman Nour^a ; Ibrahim Eldosoky^g
• 关键词：HCV ; Diagnosis ; HCV-NS4 antigen ; Liver pathologies
• 刊名：Archives of Medical Research
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：43
• 期：7
• 页码：555-562
• 全文大小：374 K

文摘

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Background and Aims

Hepatitis C virus (HCV) has emerged as the major pathogen of liver disease worldwide. The aim of this study was to quantitate and evaluate the performance of HCV-NS4 antigen as an alternative approach for confirmation of viremia.

Methods

Detection of HCV-NS4 was assessed in 883 patients with chronic hepatitis C. Areas under the ROC curves (AUC) were used to assess and compare diagnostic accuracy of ELISA for HCV-NS4 with quantitative HCV-RNA as a gold standard.

Results

HCV-NS4 was identified at 27 kDa using Western blot. AUC for HCV-NS4 detection was 0.95 for all patients with different liver pathologies: 0.93 for liver fibrosis (LF), 0.95 for liver cirrhosis (LC) and 0.98 for hepatocellular carcinoma (HCC). The mean ¡À SD (¦Ìg/mL) of HCV-NS4 in LF was 94.2 ¡À 55.6; in LC was 99.3 ¡À 64.8 and in HCC was 124.9 ¡À 70.3.

Conclusions

HCV-NS4 antigen detection using ELISA is a reliable test in the confirmation of HCV infection.