Cellular immunogenicity of a multi-epitope peptide vaccine candidate based on hepatitis C virus NS5A, NS4B and core proteins in HHD-2 mice

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• 作者：Xiao-jun Huang^a ; Xin Lü ; ^b ; Ying-feng Lei^b ; Jing Yang^b ; Min Yao^b ; Hai-yun Lan^b ; Jian-min Zhang^b ; Zhan-sheng Jia^c ; Wen Yin^d ; Zhi-kai Xu^b ; ^{zhikaixu06@gmail.com}
• 关键词：ALT ; alanine aminotransferase ; DMSO ; dimethyl sulfoxide ; ELISPOT ; enzyme-linked immunospot ; HCV ; hepatitis C virus ; IFA ; incomplete Freund's adjuvant ; CFA ; complete Freund's adjuvant ; IFN-¦Á ; interferon-¦Á ; IFN-¦Ã ; interferon-¦Ã ; OD ; optical density ; LDH
• 刊名：Journal of Virological Methods
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：189
• 期：1
• 页码：47-52
• 全文大小：517 K

文摘

To develop a vaccine against hepatitis C virus (HCV), a multi-epitope peptide was synthesized from nonstructural proteins containing HLA-A2 epitopes inducing mainly responses in natural infection. The engineered vaccine candidate, VAL-44, consists of multiple epitopes from the HCV NS5A, NS4B and core proteins. Immunization with the VAL-44 peptide induced higher CTL responses than those by the smaller VL-20 peptide. VAL-44 induced antigen-specific IFN-¦Ã-producing CD4+ T cells and CD8+ T cells. VAL-44 elicited a Th1-biased immune response with secretion of high amounts of IFN-¦Ã and IL-2, compared with VL-20. These results suggest that VAL-44 can elicit strong cellular immune responses. The VAL-44 peptide stimulated IFN-¦Ã production from viral-specific peripheral blood mononuclear cells (PBMCs) of patients infected with HCV. These results suggest that VAL-44 could be developed as a potential HCV multi-epitope peptide vaccine.