The 28-day exposure to fenpropathrin decreases locomotor activity and reduces activity of antioxidant enzymes in mice brains

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• 作者：Barbara Nieradko-Iwanicka ; ^{bnieradkoiwanicka@umlub.pl" class="auth_mail" title="E-mail the corresponding author} ; Andrzej Borzęcki
• 关键词：Fenpropathrin ; Insecticide ; Locomotor activity ; Antioxidant enzymes
• 刊名：Pharmacological Reports
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：68
• 期：2
• 页码：495-501
• 全文大小：520 K

文摘

Fenpropathrin (Fen) is a pyrethroid (Pyr) insecticide. Pyrs are used in veterinary medicine, in agriculture and for domestic purposes. As their use increases, new questions about their side effects and mode of action in non-target organisms arise.

The objective of this work was to characterize dose–response relationship for in vivo motor function and memory in mice exposed to Fen for 28 days and to assess its influence on activity of antioxidant enzymes in mice brains.

Methods

The experiment was performed using 64 female mice. Fen at the dose of 11.9 mg/kg of body mass, 5.95 mg/kg or 2.38 mg/kg was administered ip to the mice for 28 consecutive days. Motor function and spatial working memory were tested on days 7, 14 and 28. On day 29, the animals were sacrificed and brains were used to determine activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx).

Results

Fen significantly decreased locomotor activity in mice receiving the highest dose at every stage of the experiment. Lower doses reduced locomotion on days 7 and 14. Fen did not produce memory impairment. A decrease in activities of SOD and GPx was recorded in mice brains.

Conclusions

The decrease of SOD activity in mice brains results from direct inhibition of the enzyme by Fen and/or increased utilization due to excessive free radical formation in conditions of Fen-induced oxidative stress. The reduction in GPx activity is probably due to limited glutathione availability. The reduced locomotor activity is a behavioral demonstration of Fen-induced damage in the dopaminergic system.