- 作者：Antonia Kolokythas ; DDS? ; akolokythas@yahoo.com ; Maria Karas ; BS&dagger ; ; Thomas Sarna ; DDS&Dagger ; ; William Flick ; DDS§ ; ; Michael Miloro ; DMD ; MD¡¬
- 刊名：Journal of Oral and Maxillofacial Surgery
- 出版年：2012
- 出版时间：May, 2012
- 年：2012
- 卷：70
- 期：5
- 页码：1070-1080
- 全文大小：694 K
Purpose
The objective of the present study was twofold: first, to assess aspirates for use in cytokine profiling and second, to initiate pilot analyses to determine whether the cytokine profiling can serve as an aid in the diagnosis of jaw lesions.Materials and Methods
The aspirates from 12 benign odontogenic cysts and tumors of the jaw were collected and randomized, and a formal incisional biopsy was performed to establish the tissue diagnosis. The biopsies revealed keratocystic odontogenic tumor, ameloblastoma, and dentigerous cyst. The cystic aspirate was analyzed using the Q-Plex Human Cytokine Screen to detect cytokine expression and determine the level of expression for each pathologic entity. An array of 16 cytokines was investigated, including interleukin (IL)-1¦Á, IL-1¦Â, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-15, IL-17, IL-23, interferon-¦Ã, tumor necrosis factor (TNF)-¦Á, and TNF-¦Â. Tables were developed to determine the ratio of expression for the candidate cytokine pairs that were differentially expressed among the 3 pathologic entities encountered. One-way analysis of variance was used to search for significant differences in the ratio of expression of the candidate pairs among the 3 entities.
Results
Cytokines expressed by the 3 distinct jaw lesions were detected in the aspirate without the need for tissue biopsy. Cytokine profiling of these entities is possible owing to differential expression of the various cytokines studied. The ratio of expression was significant (P < .05) for 15 pairs of cytokines: IL-5/IL-1¦Á, IL-4/IL-2, IL-8/IL-4, TNF-¦Â/IL-6, IL-23/IL-6, TNF-¦Á/IL-23, TNF-¦Á/TNF-¦Â, TNF-¦Á/IL-8, TNF-¦Â/IL-5, TNF-¦Â/TNF-¦Á, TNF-¦Â/IL-13, IL-12/IL-23, IL-13/IL-15, IL-15/IL-2, and IL-6/IL-2. A comparison of the mean values indicated a ¡°high/low¡± expression value for each lesion type for the 15 cytokine pairs.
Conclusions
Cytokines, expressed by the 3 groups of jaw lesions, can be detected in the cystic aspirate, and a comparison of the ratio of the expression of the aspirates demonstrated a differential expression pattern of cytokines among the 3 groups. These ratios could assist in establishing a prompt and accurate diagnosis of lesions that might be difficult to discern clinically and radiographically. The use of a simple, minimally invasive aspiration procedure can help to establish an accurate diagnosis.